The readseq jar file is located at /usr/share/java/readseq/readseq.jar Normally readseq is used by typing a command such as: java -cp /usr/share/java/readseq/readseq.jar run myfile -f 12 -o outfile For the arch installation, a script has been placed at /usr/bin/readseq that passes command line args to the readseq jar file. So, to run the java command given above, you just need to type: readseq run myfile -f 12 -o outfile If you run /usr/bin/readseq without command line arguments, readseq will open as a gui application.